NCERT Solutions for Class 12 Biology Chapter 6 - Molecular Basis of Inheritance

NCERT Solutions for Class 12 Biology Chapter 6 – Free PDF Download

*According to the CBSE Syllabus 2023-24, this chapter has been renumbered as Chapter 5.

Looking for NCERT Solutions for Class 12 Biology Chapter 6 Molecular Basis of Inheritance? Look no further as BYJU’S offers solutions to all the chapters of NCERT Solutions for Class 12 Biology in detail here. These are according to the latest CBSE Syllabus 2023-24. This chapter provides students with insights into the structure of DNA, its replication, the process of synthesising RNA from DNA (transcription process), the genetic code which determines the sequences of amino acids in proteins, the phenomena of protein synthesis and the elementary basis of their regulation.

Also, with the help of NCERT Solutions for Class 12 Biology, students can understand other subsequent sections, like why DNA is the most abundant genetic material and what its relationship is with RNA. Towards the end, the chapter also discusses the essentials of human genome sequencing and its consequences. Access the NCERT Solutions for Class 12 Biology PDF of this chapter from the link given below.

NCERT Solutions for Class 12 Biology Chapter 6 – Molecular Basis of Inheritance

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Access Answers to Biology NCERT Chapter 6 – Molecular Basis of Inheritance

1. Group the following as nitrogenous bases and nucleosides:

Adenine, Cytidine, Thymine, Guanosine, Uracil and Cytosine.

Solution:

Following is the grouping:

2. If a double-stranded DNA has 20 per cent of cytosine, calculate the per cent of adenine in the DNA.

Solution:

As per Chargaff’s rule, DNA molecules are required to have an equal ratio of purine (adenine and guanine) and pyridine (cytosine and thymine). This is to say that the number of adenine molecules is equivalent to the cytosine molecule.

Percentage of adenosine = percentage of thymine

Percentage of guanine = percentage of cytosine

Hence, according to the law, if the double-stranded DNA has 20% of cytosine, it should have 20% of guanine. Therefore, the percentage of G + percentage of C = 40%

The other 60% indicates both A + T percentage molecules. As adenine and thymine are always found in equal numbers, the adenine content is 30%.

3. If the sequence of one strand of DNA is written as follows:

5′ -ATGCATGCATGCATGCATGCATGCATGC-3′

Write down the sequence of the complementary strand in a 5’→3′ direction.

Solution:

With regard to base sequence, DNA strands are complementary to each other. Therefore, if the sequence of one strand of DNA is written as:

5′ -ATGCATGCATGCATGCATGCATGCATGC-3′

The sequence of the complementary strand in 3′ -> 5′ is as follows:

3′ – TACGTACGTACGTACGTACGTACGTACG – 5′

Subsequently, the sequence of the complementary strand in 5’→3′ direction is written as:

5′ – GCATGCATGCATGCATGCATGCATGCAT – 3′

4. If the sequence of the coding strand in a transcription unit is written as follows:

5′ -ATGCATGCATGCATGCATGCATGCATGC-3′

Write down the sequence of mRNA.

Solution:

In a transcription unit, if the coding strand is as follows:

5′ -ATGCATGCATGCATGCATGCATGCATGC-3′

Then the sequence that codes for nothing are the same as mRNA, except that in mRNA, uracil replaces thymine. Hence, the mRNA sequence is as written below:

5′ -AUGCAUGCAUGCAUGCAUGCAUGCAUGC-3′

5. Which property of DNA double helix led Watson and Crick to hypothesise a semi-conservative mode of DNA replication? Explain.

Solution:

Watson and Crick noted that the two DNA strands are anti-parallel, complementary to each other with regards to their base sequences which facilitates each strand to serve as a template to synthesise a new strand. This organisation of the DNA molecule furthered the hypothesis that the replication of DNA is semi-conservative. In other words, the double-stranded DNA molecule splits, and in turn, each of the separated strands serves as a template to synthesise a new complementary strand. Subsequently, each DNA molecule will have one parental strand and a newly synthesised daughter strand. As only one parental strand is conserved in each of the daughter molecules, the mode of replication is termed as semi-conservative.

The sequence of bases in the template strand because of complementary base pairing governs the sequence of bases in the daughter strand. Subsequently, this property of DNA led Watson and Crick to hypothesise the semi-conservative mode of replication.

6. Depending upon the chemical nature of the template (DNA or RNA) and the nature of nucleic acids synthesised from it (DNA or RNA), list the types of nucleic acid polymerases.

Solution:

The list includes two different types of nucleic acid polymerases:

• DNA-dependent DNA polymerases
• DNA-dependent RNA polymerases
• RNA-dependent DNA polymerases
• RNA-dependent RNA polymerases

To synthesise a new strand, the DNA-dependent DNA polymerases use a DNA template. But, DNA-dependent RNA polymerases utilise a DNA template strand to synthesise RNA.

7. How did Hershey and Chase differentiate between DNA and protein in their experiment while proving that DNA is the genetic material?

Solution:

To prove DNA is the genetic material, Hershey and Chase studied and worked on bacteriophage and E.Coli. To label the protein coat and DNA of the bacteriophage, they used different radioactive isotopes. In a medium containing radioactive phosphorous (32P), they cultivated some bacteriophages to detect DNA and a few more on a medium comprising radioactive sulphur (35S) to detect protein. The labelled radioactive phages were then made to infect the bacteria – E.coli. Once infected, the protein coat of the bacteriophage was segregated from the bacterial cell by mixing and then subject to the centrifugation process.

It was observed that, in the supernatant, as the protein coat was lighter, the infected bacteria got settled at the bottom of the centrifuge tube. In case I – the supernatant was observed to be radioactive, indicating that the protein did not enter the bacterial cell when infected. But in case II – the bacterial cells were radioactive as they possess radioactive DNA. Thus, it was proved that DNA is a genetic material as it was transferred from viruses to bacteria.

8. Differentiate between the following:

(a) Repetitive DNA and satellite DNA

(b) mRNA and tRNA

(c) Template strand and coding strand

Solution:

(a) Repetitive DNA and Satellite DNA

(b) mRNA and tRNA

(c) Template Strand and Coding Strand

9. List two essential roles of the ribosome during translation.

Solution:

Two essential roles of the ribosome during translation are as given below:

• Ribosomes are sites where the synthesis of proteins occurs from individual amino acids. It consists of two subunits – the larger subunit serves as an amino acid binding site, whereas the smaller subunit attaches to the mRNA forming a protein-synthesising complex
• Since the large subunit of the ribosome has two different sites to attach to tRNA, it facilitates amino acids to come closer for peptide bond formation. Also, the ribosome behaves as a catalyst for the formation of the peptide bond. Example – 23s r-RNA acts as a ribozyme in bacteria

10. In the medium where E. coli was growing, lactose was added, which induced the lac operon. Then, why does the lac operon shut down sometime after the addition of lactose in the medium?

Solution:

A segment of DNA which comprises three adjacent structural genes – a promoter gene, an operator gene, and a regulator gene is known as a lac operon. It is functional in a coordinated manner in order to metabolise lactose into galactose and glucose. Lactose serves as an inducer in the lac operon, which binds to the repressor, inactivating it. Once the lactose is bound to the repressor, RNA polymerase binds to the area of the promoter. Therefore, the structural genes express their product, and corresponding enzymes are yielded. Lactose is metabolised due to the action of enzymes into galactose and glucose. Later on, the level of inducer decreases as enzymes completely metabolise them, which results in the synthesis of the repressor from the regulator gene. Thus, the repressor is bound to the operator gene, restricting RNA polymerase from transcribing the operon; subsequently, transcription is ceased. This kind of regulation is termed negative regulation.

11. Explain (in one or two lines) the function of the followings:

(a) Promoter

(b) tRNA

(c) Exons

Solution:

(a) Promoter

The section of DNA which aids in initiating the transcription process and serving as a binding site for RNA polymerase is the promoter

(b) tRNA

The transfer RNA or tRNA is a small clover-shaped RNA that reads the genetic code that is located on mRNA. It conveys particular amino acids to the mRNA ribosome while proteins are translated. Specific tRNAs are present for specific amino acids.

(c) Exons

In eukaryotes, these are coding sequences of DNA which transcribe proteins. In between, exons comprise long non-coding sections of DNA known as introns.

12. Why is the Human Genome project called a mega project?

Solution:

The Human Genome project was ought to be a mega project as the scale and the goals of the project were humongous. It had a goal to sequence every base pair located in the human genome, which took around 13 years to complete and was accomplished in 2003. This large-scale project intended to develop new technologies and produce new information in the stream of genomic studies. Subsequently, it unlocked the scope for several new areas and possibilities, such as in the stream of biotechnology, genetics, medical sciences etc., which hints at comprehending different aspects of human biology.

13. What is DNA fingerprinting? Mention its application.

Solution:

The technique of DNA fingerprinting is helpful in identifying and analysing variations in different individuals at the DNA level. It is based on the principle of variability and polymorphism in DNA sequences.

Its applications are as follows:

• To identify potential crime suspects in forensic science
• Helpful in establishing family and paternity relationships
• Useful in identifying and preserving the commercial varieties of livestock and crops
• Useful to discover and know more about the evolutionary history of an entity, thus tracing the linkages between different entities.

14. Briefly describe the following:

(a) Transcription

(b) Polymorphism

(c) Translation

(d) Bioinformatics

Solution:

(a) Transcription

It is the process of synthesising RNA from a DNA template. A section of DNA gets duplicated into mRNA in the process. The transcription process originates from the promoter region of the template DNA and ends at the terminator region. The section of DNA between these two regions is termed a transcription unit. The transcription process necessitates a DNA template, RNA polymerase enzyme, four types of ribonucleotides and a few cofactors, namely Mg2+.

Three significant events occurring during the transcription process are:

• Initiation
• Elongation
• Termination

The process of transcription is initiated when the DNA-dependent RNA polymerase and several initiation factors bind at the promoter region of the template strand at the double-stranded DNA. The RNA polymerase passes along the DNA, causing the DNA duplex to unwind into two distinct strands. One of the strands is known as the sense strand, which serves as a template for mRNA synthesis. The RNA polymerase enzyme uses nucleoside triphosphates (dNTPs) as raw material to polymerize them to form mRNA as per the complementary bases positioned in the template DNA. This phenomenon of the opening of the helix and elongation of the polynucleotide chain lasts until the enzyme arrives at the terminator region. Once the RNA polymerase arrives at the terminator section, the freshly produced mRNA transcripted in addition to the enzyme is liberated. Additionally, another factor known as the terminator factor is essential for the transcription process to end.

(b) Polymorphism

It is a form of genetic variation wherein different nucleotide sequences can be present at different sites in a molecule of DNA. There is a high frequency of this heritable mutation to be observed in a population which emerges as a result of mutation either in the germ cells or somatic cells. The germ cell mutation can be passed from parents to offspring, which leads to the accumulation of different mutations in a population, causing variation and polymorphism in the population. This has a crucial role to play in the evolution and speciation process.

(c) Translation

It is the process wherein amino acids are polymerized for the formation of a polypeptide chain, a ribosome, by reading an mRNA molecule. In mRNA, the triplet sequence of base pairs defines the order and sequence of amino acids in a polypeptide chain.

This phenomenon includes three steps:

• Initiation
• Elongation
• Termination

The tRNA gets charged during the initiation of the translation when the amino acids bind to it with the help of ATP. The initiation code – AUG that is located on the mRNA is identified only by the charged tRNA. For the process of translation, the ribosome acts as an actual site, containing two distinct sites in a large subunit to attach to the following amino acids. The smaller subunit of the ribosome is bound to the mRNA at AUG (initiation code), subsequently by the large subunit. The process of translation is then said to be initiated.

In the elongation process, the ribosome passes one codon downstream with the mRNA in order to leave some space to bind another charged tRNA. Amino acid brings tRNA, which gets linked with the former amino acid via a peptide bond. The process continues leading to the formation of a polypeptide chain. When the ribosome arrives, one or more STOP codons (UAA, UGA, and UAG). The phenomena of translation end, polypeptide chain releases and the ribosomes get detached from mRNA.

(d) Bioinformatics

It is the application of statistical and computational methodologies to the molecular biology stream. It explains practical issues that arise from the analysis and management of biological data. The stream of bioinformatics developed after accomplishing the human genome project as a huge amount of data was produced during the process, which has to be stored and managed for easy access and analysis for later use. Thus, bioinformatics includes creating biological databases which store huge amounts of data on biology. It comes up with a few tools for efficient and easy access to data which can be used. Also, bioinformatics brought in new algorithms and statistical techniques to figure out the dynamics between data, to predict the structure of protein and their functionalities and to group protein sequences into their associated families.

NCERT Solutions for Class 12 Biology Chapter 6 – Molecular Basis of Inheritance

Chapter 6 Molecular Basis of Inheritance of Class 12 Biology, is well-structured in accordance with CBSE Syllabus for 2023-24. The NCERT Solutions for Class 12 Biology for this chapter is categorised under the Unit – Genetics and Evolution. The chapter covers important topics such as DNA, RNA, the process of replication etc.

Important concepts mentioned in the chapter are given below:

• The DNA
• The Search for Genetic Material
• RNA World
• Replication
• Transcription
• Genetic Code
• Translation
• Regulation of Gene Expression
• Human Genome Project
• DNA Fingerprinting

Key Features of NCERT Solutions Class 12 Biology Chapter 6 Molecular Basis of Inheritance

• Easy to understand
• Solutions developed by subject matter experts
• It enhances the conceptual knowledge of the students
• NCERT Solutions are also helpful for competitive exams
• Readily available and easily accessible

BYJU’S NCERT Solutions is one of the best with its easy-to-understand and precise solutions. The solutions are well-researched and structured in a stepwise manner by subject experts at BYJU’S. The NCERT Solutions are available for Classes 1 to 12 and for a wide range of subjects too. Moreover, students can access these resources for free.